{"id":3718,"date":"2023-03-13T01:49:37","date_gmt":"2023-03-12T20:19:37","guid":{"rendered":"https:\/\/vajiramandravi.com\/current-affairs\/?p=3718"},"modified":"2025-03-26T17:23:54","modified_gmt":"2025-03-26T11:53:54","slug":"what-is-fluorescence-microscopy","status":"publish","type":"post","link":"https:\/\/vajiramandravi.com\/current-affairs\/what-is-fluorescence-microscopy\/","title":{"rendered":"What is Fluorescence Microscopy?"},"content":{"rendered":"<h2><strong>About Fluorescence Microscopy:<\/strong><\/h2>\n<ul>\n<li>A fluorescence microscope <strong>views an object by studying how it re\u00acemits light that it has absorbed<\/strong>, i.e., how it fluoresces. This is its basic principle.<\/li>\n<li><strong>How it works?<\/strong>\n<ul>\n<li>The <strong>object is illuminated<\/strong> with light of a <strong>specific wavelength<\/strong>.<\/li>\n<li><strong>Particles in the object absorb this light and re\u00acemit<\/strong> it at a higher wavelength (i.e., different color). <strong>These particles are called fluorophores<\/strong>.<\/li>\n<li><strong>The object is infused with fluorophores<\/strong> before being placed under the microscope.<\/li>\n<li><strong>When the fluorophores fluoresce, a fluorescent microscope can track them<\/strong> as they move inside the object, <strong>revealing the object\u2019s internal shape<\/strong> and other characteristics.<\/li>\n<\/ul>\n<\/li>\n<li>Scientists have developed <strong>different fluorophores to identify<\/strong> and study <strong>different entities<\/strong>, from specific parts of the DNA to protein complexes.<\/li>\n<li><strong>Applications of Fluorescence Microscope:<\/strong>\n<ul>\n<li>It is <strong>used to image specific features<\/strong> of small specimens such as microbes.<\/li>\n<li>It is also used to <strong>visually enhance 3-D features at small scales<\/strong>.<\/li>\n<li>It <strong>allows<\/strong> the use of <strong>multicolor staining<\/strong>, <strong>labeling of structures within cell<\/strong>s, and the <strong>measurement of the physiological state of a cell<\/strong>.<\/li>\n<li>It is the most popular method for studying the dynamic behavior exhibited in live-cell imaging.<\/li>\n<li>Different molecules can now be stained with different colors, allowing <strong>multiple types of molecules to be tracked simultaneously.<\/strong><\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<h3>What is Optical Microscopy?<\/h3>\n<ul>\n<li>An optical microscope, also known as a <strong>light microscope<\/strong>, <strong>views an object by studying how it absorbs, reflects, or scatters visible light.<\/strong><\/li>\n<li>It <strong>uses one or a series of lenses to magnify images<\/strong> of small samples <strong>with visible light<\/strong>.<\/li>\n<li>The lenses are placed between the sample and the viewer\u2019s eye to magnify the image so that it can be examined in greater detail.<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<hr \/>\n<h3>Q1)\u00a0What is Transmission electron microscopy (TEM)?<\/h3>\n<p>Transmission electron microscopes (TEM) are microscopes that use a particle beam of electrons to visualize specimens and generate a highly-magnified image. TEMs can magnify objects up to 2 million times.<\/p>\n<p><strong>Source:\u00a0<\/strong><a href=\"https:\/\/www.thehindu.com\/sci-tech\/science\/scientists-devise-glowscope-to-bring-fluorescent-microscopy-to-schools\/article66607143.ece\" target=\"_blank\" rel=\"nofollow noopener\"><u>Scientists devise \u2018glowscope\u2019 to bring fluorescent microscopy to schools<\/u><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>A fluorescence microscope views an object by studying how it re\u00acemits light that it has absorbed.<\/p>\n","protected":false},"author":5,"featured_media":3719,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[1],"tags":[],"class_list":{"0":"post-3718","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-upsc-prelims-current-affairs","8":"no-featured-image-padding"},"acf":[],"_links":{"self":[{"href":"https:\/\/vajiramandravi.com\/current-affairs\/wp-json\/wp\/v2\/posts\/3718","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/vajiramandravi.com\/current-affairs\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/vajiramandravi.com\/current-affairs\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/vajiramandravi.com\/current-affairs\/wp-json\/wp\/v2\/users\/5"}],"replies":[{"embeddable":true,"href":"https:\/\/vajiramandravi.com\/current-affairs\/wp-json\/wp\/v2\/comments?post=3718"}],"version-history":[{"count":0,"href":"https:\/\/vajiramandravi.com\/current-affairs\/wp-json\/wp\/v2\/posts\/3718\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/vajiramandravi.com\/current-affairs\/wp-json\/wp\/v2\/media\/3719"}],"wp:attachment":[{"href":"https:\/\/vajiramandravi.com\/current-affairs\/wp-json\/wp\/v2\/media?parent=3718"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/vajiramandravi.com\/current-affairs\/wp-json\/wp\/v2\/categories?post=3718"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/vajiramandravi.com\/current-affairs\/wp-json\/wp\/v2\/tags?post=3718"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}