{"id":49677,"date":"2025-06-10T14:00:11","date_gmt":"2025-06-10T08:30:11","guid":{"rendered":"https:\/\/vajiramandravi.com\/current-affairs\/?p=49677"},"modified":"2025-08-22T11:35:10","modified_gmt":"2025-08-22T06:05:10","slug":"magnetic-isolation-and-concentration-cryo-electron-microscopy","status":"publish","type":"post","link":"https:\/\/vajiramandravi.com\/current-affairs\/magnetic-isolation-and-concentration-cryo-electron-microscopy\/","title":{"rendered":"Magnetic Isolation and Concentration Cryo-Electron Microscopy"},"content":{"rendered":"<h2><strong>Magnetic Isolation and Concentration Cryo-Electron Microscopy \u00a0Latest News<\/strong><\/h2>\n<p>Researchers in the U.S. have created Magnetic Isolation and Concentration cryo-electron microscopy (MagIC) which can study samples 100x more dilute than before.<\/p>\n<h2><strong>About Magnetic Isolation and Concentration Cryo-Electron Microscopy<\/strong><\/h2>\n<ul>\n<li>It helps researchers to\u00a0<strong>study samples 100 times<\/strong>\u00a0more dilute than before.<\/li>\n<li>It is a technique\u00a0<strong>enabling direct structural analysis<\/strong>\u00a0of targets captured on magnetic beads, thereby reducing the targets\u2019 concentration requirement to &lt;0.0005 mg\/mL.<\/li>\n<\/ul>\n<h2><strong>Working of Magnetic Isolation and Concentration Cryo-Electron Microscopy\u00a0<\/strong><\/h2>\n<ul>\n<li>It uses\u00a0<strong>magnetic beads (50-nm size beads)<\/strong>\u00a0to hold molecules in place.<\/li>\n<li>This\u00a0<strong>greatly reduces<\/strong>\u00a0the amount of protein needed in the initial sample.<\/li>\n<li>A refined analysis method\u00a0<strong>called\u00a0<\/strong>Duplicated Selection To Exclude Rubbish (DuSTER) was designed in parallel to help capture the structure of\u00a0<strong>smaller molecules.<\/strong><\/li>\n<li>MagIC-cryo-EM and DuSTER will allow scientists to use cryo-EM to examine the structure of proteins which are smaller, challenging to isolate or present in minute quantities inside cells.<\/li>\n<li><strong>Application:<\/strong>\u00a0The knowledge gained from these findings could help us better understand a wide\u00a0<strong>range of biological processes.<\/strong><\/li>\n<\/ul>\n<h3><strong>Key Facts about Cryo-Electron Microscopy \u00a0(Cryo-EM)<\/strong><\/h3>\n<ul>\n<li>It is a technique that allows researchers to\u00a0<strong>directly visualize proteins.<\/strong><\/li>\n<li>The approach first requires\u00a0<strong>flash-freezing a sample at extremely low temperatures<\/strong>, and then sending a beam of electrons through the specimen from several angles.<\/li>\n<li>The resulting series of two-dimensional images are analysed using advanced algorithms to construct a final three-dimensional protein model.<\/li>\n<li><strong>Limitations:<\/strong>\u00a0Despite its power, cryo-EM also has limitations, including requiring very large quantities of protein in the initial sample. The algorithms currently available for Cryo-EM analyses also struggle to determine\u00a0<strong>the structure of smaller molecules.<\/strong><\/li>\n<\/ul>\n<p><strong>Source:\u00a0<\/strong><a href=\"https:\/\/www.thehindu.com\/sci-tech\/science\/magical-new-technique-brings-very-dilute-samples-into-focus\/article69669270.ece\" target=\"_blank\" rel=\"nofollow noopener\">TH<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Magnetic Isolation and Concentration cryo-electron microscopy helps researchers to study samples 100 times more dilute than before.<\/p>\n","protected":false},"author":8,"featured_media":49693,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[1],"tags":[775,21,22,23],"class_list":{"0":"post-49677","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-upsc-prelims-current-affairs","8":"tag-magnetic-isolation-and-concentration-cryo-electron-microscopy","9":"tag-prelims-pointers","10":"tag-upsc-current-affairs","11":"tag-upsc-prelims-current-affairs","12":"no-featured-image-padding"},"acf":[],"_links":{"self":[{"href":"https:\/\/vajiramandravi.com\/current-affairs\/wp-json\/wp\/v2\/posts\/49677","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/vajiramandravi.com\/current-affairs\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/vajiramandravi.com\/current-affairs\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/vajiramandravi.com\/current-affairs\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/vajiramandravi.com\/current-affairs\/wp-json\/wp\/v2\/comments?post=49677"}],"version-history":[{"count":0,"href":"https:\/\/vajiramandravi.com\/current-affairs\/wp-json\/wp\/v2\/posts\/49677\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/vajiramandravi.com\/current-affairs\/wp-json\/wp\/v2\/media\/49693"}],"wp:attachment":[{"href":"https:\/\/vajiramandravi.com\/current-affairs\/wp-json\/wp\/v2\/media?parent=49677"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/vajiramandravi.com\/current-affairs\/wp-json\/wp\/v2\/categories?post=49677"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/vajiramandravi.com\/current-affairs\/wp-json\/wp\/v2\/tags?post=49677"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}